Chromatin Immunoprecipitation Assays: Methods and Protocols (Methods in Molecular Biology)




E-Book Name Title for Download :Chromatin Immunoprecipitation Assays: Methods and Protocols (Methods in Molecular Biology) (Format : pdf)

Plot : Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNAbinding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cuttingedge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genomewide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitationbased DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Written in the highly successful Methods in Molecular Biology series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easytouse, Chromatin Immunoprecipitation Assays: Methods and Protocols is a timely guide for all those who wish to further the development of this powerful tool and continue delving into the incredible complexity of the cells biology.



Author(s) Book :Philippe Collas
E-Book Categories :Biochemistry; Genetics; Cell Biology; Biochemistry; Genetics
Publish Year :2009 Publisher : Humana Press
You can check by ISBN10: 1603274138; ISBN13: 9781603274135
Pages :258

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